ENBIS-8 in Athens

21 – 25 September 2008 Abstract submission: 14 March – 11 August 2008

23 September 2008, 11:35 – 11:40

Abstract

Submitted by
Rozina Vavetsi
Authors
Rozina Vavetsi Department of Experimental Pharmacology, School of Medicine, University of Athens and T.E.I Piraeus/University of Paisley, Scotland 75 M.Asias St., Goudi 15669 Athens, Greece E-mail: rvavetsi@med.uoa.gr Chrysanthi Papanastasopoulou Department of Experimental Pharmacology, School of Medicine, University of Athens 75 M.Asias St., Goudi 15669 Athens, Greece E-mail: chpapan@med.uoa.gr Georgia Dougekou Department of Experimental Pharmacology, School of Medicine, University of Athens 75 M.Asias St., Goudi 15669 Athens, Greece E-mail: ssapounas@gmail.com George J. Besseris T.E.I Piraeus/University of Paisley, Scotland, Argirokastrou 30 St., Drosia 14572 Athens, Greece E-mail: besseris@teipir.gr Nikolaos M. Sitaras Department of Experimental Pharmacology, School of Medicine, University of Athens 75 M.Asias St., Goudi 15669 Athens, Greece E-mail: nsitar@med.uoa.gr
Abstract
Glycosaminoglycans (GAGs), a class of complex polysaccharides of the extracellular environment have been recognized to participate in numerous physiological and pathological processes. Dermatan sulphate, the predominant glycan in skin, is particularly attractive because it is expressed in many mammalian tissues and has been implicated in tumorigenesis, would repair, infections and fibrosis. Human nails – a special epithelial structure with continuously produced cells that become keratinized and eventually desquamated- have not yet been studied for their glycosaminoglycan content.
The aim of the present study was a) to investigate the content of glycosaminoglycans in human nails and optimize the extraction laboratory protocol so the maximum of them is liberated, and b) study the presence of dermatan sulphate in this material.
Unpolished human nails were obtained from 30 healthy individuals and GAGs were sequentially extracted with guanidinium chloride (Gu-HCl). To optimize the extraction protocol the Taguchi method was applied. Cellulose acetate electrophoresis was performed for the qualitative identification of GAGs. Enzymatic treatment with specific lyases followed, to study the presence of dermatan sulphate in this material.
The results indicated the best combination of the extraction parameters that liberated the maximum concentration of GAGs from nails, being 116 ± 15.8 μg/g of tissue. Cellulose acetate electrophoresis after treatment with specific enzymes revealed a metachromatic band with mobility identical to dermtan’ s sulfate

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